Coding

Part:BBa_K3651005:Design

Designed by: Zoe Hsieh, Maria Lyons, Josh Dayie, Max Swann   Group: iGEM20_Baltimore_BioCrew   (2020-09-26)


Codon optimized tonB gene for Synechococcus sp. CB0101 with restriction sites removed


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1828
    Illegal SpeI site found at 1918
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1828
    Illegal SpeI site found at 1918
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1828
    Illegal BglII site found at 2364
    Illegal BamHI site found at 2082
    Illegal XhoI site found at 2020
    Illegal XhoI site found at 2368
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1828
    Illegal SpeI site found at 1918
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1828
    Illegal SpeI site found at 1918
    Illegal NgoMIV site found at 756
    Illegal NgoMIV site found at 2504
    Illegal AgeI site found at 1537
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 214


Design Notes

NA


Source

Synechocystis sp. PCC 6803


References